Antibacterial effects of bio-inspired nanostructured materials

Several properties of bio-inspired surfaces like chemical composition, surface topography, surface hydrophilicity and even surface charge could influence bacterial adhesion to implant materials. Therefore, a nanostructured surface is being investigated to avoid bacterial colonization by their physico-mechanical and chemical aspects. Both smooth and rough-surfaced titanium (PT, SLA) and zirconia (M and ZLA) surfaces were used as controls. Titanium SLA was modified by two-step-etching to create nanostructured surface. Antibacterial properties of the materials were tested by adhesion of Porphyromonas gingivalis (ATCC 33277). The vitality of bacteria was assessed by Live/Dead BacLightTM Bacterial Viability Kit or by conventional culturing on Columbia blood agar. Conventional culturing revealed reduction of bacteria on nanostructured titanium (5.27±0.8 x 10 CFU/mm) in comparison to rough-surfaced control materials (ZLA 6.16 ±4.86 x 10 and SLA 1.53±0.75 x 10 CFU/mm). However, smooth-surfaced control materials (M 2.25±0.84 x 10 and PT 6.63±5.77 x 10 CFU/mm) showed similar results to the nanostructured material. Live/dead staining demonstrated the antimicrobial efficacy of the nanostructured material revealing reduction of vital bacteria population up to 70%. This effect was not observed on the control materials (bacterial vitality ≥95%). In conclusion, nanostructured titanium surface shows a reduction of vital bacteria. Therefore, bio-inspired nanostructures can modify the bacteria–titanium interaction. CONTACT Monika Astasov-Frauenhoffer [email protected] JOURNAL OF ORAL MICROBIOLOGY, 2017 SUPPLEMENT, 1325241 https://doi.org/10.1080/20002297.2017.1325241 © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.